[Japan Atomic Energy Agency - JAEA (Japan) Research and Development of Crystal Purification for Product of Uranium Crystallization ProcessĮnergy Technology Data Exchange (ETDEWEB) The crystals diffracted to a resolution of 1.9 Ã… resolution and crystal structure determination is in progress In this paper, the expression, purification and crystallization of nsp10 in complex with nsp 16 are reported. Nsp 16 is an RNA-cap AdoMet-dependent (nucleoside-2â€²-O-)-methyltransferase that is only active in the presence of nsp10. The crystal structure of nsp 16 is unknown. This positive RNA virus encodes a large replicase polyprotein made up of 16 gene products (nsp1â€“ 16), amongst which two methyltransferases, nsp14 and nsp 16, are involved in viral mRNA cap formation. In 2003, it was responsible for a large outbreak associated with a 10% fatality rate. To date, the SARS coronavirus is the only known highly pathogenic human coronavirus. The expression, purification and crystallization of the SARS coronavirus nsp 16 RNA-cap AdoMet-dependent (nucleoside-2â€²O)-methyltransferase in complex with its activating factor nsp10 are reported. The H-1PV structure has been determined by molecular replacement and is currently being refinedĬrystallization and diffraction analysis of the SARS coronavirus nsp10â€“nsp 16 complexĭebarnot, Claire Imbert, Isabelle Ferron, FranÃ§ois Gluais, Laure Varlet, Isabelle Papageorgiou, Nicolas Bouvet, MickaÃ«l Lescar, Julien Decroly, Etienne Canard, Bruno The unit cell contained two capsids, with one capsid per crystallographic asymmetric unit.

The crystals belonged to the monoclinic space group P2 1, with unit-cell parameters a = 255.4, b = 350.4, c = 271.6 Ã…, Î² = 90.34Â°. Crystals of H-1 Parvovirus ( H-1PV), an antitumor gene-delivery vector, were obtained for DNA-containing capsids and diffracted X-rays to 2.7 Ã… resolution using synchrotron radiation. The production, purification, crystallization and crystallographic analysis of H-1 Parvovirus, a gene-therapy vector, are reported. Halder, Sujata Nam, Hyun-Joo Govindasamy, Lakshmanan Vogel, MichÃ¨le Dinsart, Christiane SalomÃ©, Nathalie McKenna, Robert Agbandje-McKenna, Mavis International Nuclear Information System (INIS) Production, purification, crystallization and structure determination of H-1 Parvovirus

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